Insulinotropic and Cytoproctective Effect of Crude Extract of Eugenia uniflora L. Leaves
Abstract
Eugenia uniflora L. leaves (“pitangueira”) are popularly used as an alternative to the treatment of diabetes mellitus. The objective of this work was to evaluate the cytotoxicity, antioxidant, cytoprotection and insulinotropic activity of crude leaf extracts of Eugenia uniflora L. on RINm5f cells. The antioxidant activity was analyzed by the Folin-Ciocalteau method; cytotoxicity and cytoprotection using the methods of MTT reduction and HE staining and the insulinotropic effect by radioimmunoassay. The aqueous extract (AQ) presented higher phenolic content than the alcoholic extract (ALC). Both showed low cytotoxicity and ALC presented a significant insulinotropic effect. AQ and ALC showed a cytoprotective effect, increasing cell viability and preventing changes in cell morphology induced by H2O2. The results obtained in this work are indicative of the potential of these extracts as coadjuvants in the prevention and treatment of diabetes mellitus.
Key words: antioxidants, cytotoxicity, cytoprotection, flavonoids, hydrogen peroxide.
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