ROOT AND SHOOT DEVELOPMENT OF CORN SEEDLINGS AS AFFECTED BY RHIZOBACTERIA
Palavras-chave:
Zea mays L, rizobactérias, sustentabilidade, raizResumo
Multifunctional microorganisms are beneficial microorganisms able of promoting plant growth through direct and indirect mechanisms. Because of this, has enormous potential for use when aiming a sustainable agriculture. The objective of this work was to determine the effect of seed inoculation with multifunctional microorganisms on the initial development of corn seedlings. The experiment was carried out under controlled conditions in a completely randomized design, with seven treatments and six replications. Treatments consisted of microbiolization of corn seeds with the rhizobacteria: 1. Pseudomonas fluorescens (BRM 32111), 2. Burkholderia pyrrocinia (BRM 32113), 3. Serratia sp. (BRM 32114), 4. Bacillus sp. (BRM 63573), 5. Azospirillum brasilense (Ab-V5), 6. Azospirillum sp. (BRM 63574) and 7. control treatment (no microorganisms). For each experimental unit, 500 mL plastic pots filled with soil and two corn seeds were used. After 14 days the seedlings were removed from the pots, set aside from the ground and photographed with a digital camera. The images presented were analyzed by WinRHIZO 2012 software to determine: total root length, root diameter, total root surface area and root volume. After this, roots and shoots of the seedlings were dried and weighed. Corn seedlings treated with the multifunctional microorganisms Bacillus sp. (BRM 63573), Serratia sp. (BRM32114) and Azospirillum sp. (BRM 63574) presented increased in the root and shoots biomass compared to untreated seeds. The microorganism Azospirillum sp. (BRM 63574) was the one that provided the highest values in the parameters of total root length, root diameter, root volume, root dry mass, shoot dry mass and total dry mass compared to the control treatment. The use of multifunctional microorganisms is a promising alternative to provide greater development of corn seedlings.
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ARSENAULT, J.-L.; POULCUR, S.; MESSIER, C.; GUAY, R. WinRHlZO™, a Root-measuring System with a Unique Overlap Correction Method. Hortscience, [S.L.], v. 30, n. 4, p. 906-906, jul. 1995. DOI: http://10.21273/HORTSCI.30.4.906D
BRACCINI, A. L., DAN, L. G. M., PICCININ, G. G., ALBRECHT, L. P., BARBOSA, M.C.; ORTIZ, A.H.T. Seed inoculation with Azospirillum brasilense, associated with the use of bioregulators in corn. Revista Caatinga, v.25, n. 2, p. 58-64, 2012.
CARDOSO, E. J. B. N.; ANDREOTE, F. D. Microbiologia do solo. 2 ed., Piracicaba: Esalq. 2016.
CARVALHO, D. D. C.; OLIVEIRA, D. F.; PASQUAL, M.; CAMPOS, V. P. Rizobactérias produtoras de promotores do crescimento de plantas. Pesquisa Agropecuária Tropical, [S. l.], v. 39, n. 4, p. 338–341, 2009.
CASSÁN, Fabricio; DIAZ-ZORITA, Martín. Azospirillum sp. in current agriculture: from the laboratory to the field. Soil Biology And Biochemistry, [S.L.], v. 103, p. 117-130, dez. 2016. DOI: http://dx.doi.org/10.1016/j.soilbio.2016.08.020.
CHAGAS, L.F Borges; CHAGAS JUNIOR, A.F; CARVALHO, M Rodrigues de; MILLER, L de Oliveira; COLONIA, B.S Orozco. Evaluation of the phosphate solubilization potential of Trichoderma strains (Trichoplus jco) and effects on rice biomass. Journal Of Soil Science And Plant Nutrition, [S.L.], v. 15, n. 3, p. 794-804, 2015. DOI: http://dx.doi.org/10.4067/s0718-95162015005000054.
CHAGAS, L. F. B.; MARTINS, A. L. L.; CARVALHO FILHO, M. R. DE; MILLER, L. DE O.; OLIVEIRA, J. C. DE; CHAGAS JUNIOR, A. F. Bacillus subtilis e Trichoderma sp. no incremento da biomassa em plantas de soja, feijão-caupi, milho e arroz. Agri-Environmental Sciences, v. 3, n. 2, p. 10-18, 2018.
CONAB - Companhia Nacional de Abastecimento. Acompanhamento da Safra Brasileira De Grãos: Décimo segundo levantamento - Safra 2018/19, v. 6, ,12, 2019.
DOURADO NETO, D.; DARIO, G.J.A.; BARBIERI, A.P.P.; MARTIN, T.N. Ação de bioestimulante no desempenho agronômico de milho e feijão. Bioscience Journal, Uberlândia, v. 30, n. Ju 2014, p. 371-379, 2014.
FAO (Organização das Nações Unidas para Alimentação e Agricultura - Divisão de Estatística). Dados de produção e colheita. 2021. Disponivel: < http://www.fao.org/faostat/en/#data/QC/visualize> Acesso: Agosto, 2021.
FERNANDES, J. P. T; NASCENTE, A. S.; FILIPPI, M. C. C.; SILVA, M. A. (2021). Upland rice seedling performance promoted by multifunctional microorganisms. Semina: Ciências Agrárias, [S.L.], v. 1, n. 42, p. 429-438, 19 jan. 2021. DOI: http://dx.doi.org/10.5433/1679-0359.2021v42n1p429.
FILIPPI, M. C. C.; SILVA, G. B.; SILVA-LOBO, V. L.; CÔRTES, M. V. C. B.; MORAES, A. J. G.; PRABHU, ANNE S. Leaf blast (Magnaporthe oryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil. Biological Control, [S.L.], v. 58, n. 2, p. 160-166, ago. 2011. DOI: http://dx.doi.org/10.1016/j.biocontrol.2011.04.016.
GLICK, B. R. Plant Growth-Promoting Bacteria: mechanisms and applications. Scientifica, [S.L.], v. 2012, p. 1-15, 2012. DOI: http://dx.doi.org/10.6064/2012/963401.
HAMEEDA, B.; HARINI, G.; RUPELA, O.P.; WANI, S.P.; REDDY, G. Growth promotion of corn by phosphate-solubilizing bacteria isolated from composts and macrofauna. Microbiological Research, [S.L.], v. 163, n. 2, p. 234-242, 2008. DOI: http://dx.doi.org/10.1016/j.micres.2006.05.009.
HUNGRIA, M. Inoculação com Azospirillum brasiliense: inovação em rendimento a baixo custo/Mariangela Hungria. – Londrina: Embrapa Soja, 2011.
KADO, C. I.; HESKETT, M. G. Selective Media for Isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology, [S.L.], v. 60, n. 6, p. 969, 1970. DOI: http://dx.doi.org/10.1094/phyto-60-969.
KAUSHAL, M. Portraying Rhizobacterial Mechanisms in Drought Tolerance: A Way Forward Toward Sustainable Agriculture. 2019. In: PGPR Amelioration in Sustainable Agriculture. p. 195-216. 2019
LAKSHMANAN, V.; SHANTHARAJ, D.; LI, G.; SEYFFERTH A. L.; SHERRIER, D. J.; BAIS, H. P. A natural rice rhizospheric bacterium abates arsenic accumulation in rice (Oryza sativa L.). Planta, [S.L.], v. 242, n. 4, p. 1037-1050, 10 jun. 2015. DOI: http://dx.doi.org/10.1007/s00425-015-2340-2.
LOPES, C. V. A.; ALBUQUERQUE, G. S. C. Agrotóxicos e seus impactos na saúde humana e ambiental: uma revisão sistemática. Saúde em Debate, [S.L.], v. 42, n. 117, p. 518-534, jun. 2018. DOI: http://dx.doi.org/10.1590/0103-1104201811714.
MAHMOOD, A.; TURGAY, O. C.; FAROOQ, M.; HAYAT, R. Seed biopriming with plant growth promoting rhizobacteria: a review. Fems Microbiology Ecology, [S.L.], v. 92, n. 8, p. 1-14, 23 maio 2016. Oxford University Press (OUP). http://dx.doi.org/10.1093/femsec/fiw112.
MILANI, R.; SANTOS, R. M.; BENTES, L. L.; KANDASAMY, S.; LAZAROVITS, G.; RIGOBELO, E. C. Bacillus subtilis isolates with different abilities to Promote plant growth in corn, cotton and soybean Crops isolation and characterization of bacterial Strains. Asian Journal of Microbiology, Biotechnology & Environmental Sciences, v. 21, n. 4, p. 827-836, 2019.
MILLÉO, M. V. R.; CRISTÓFOLI, I. Avaliação da eficiência agronômica da inoculação com AzzoFix® (Azospirillum brasilense, estirpes: abv5 e abv6) sobre a cultura do milho. Scientia Agraria, [S.L.], v. 17, n. 3, p. 14-23, 6 mar. 2017. DOI: http://dx.doi.org/10.5380/rsa.v17i3.44630
MIRANDA, R. A. Uma história de sucesso da civilização. A Granja, v. 74, n. 829, p. 24-27, 2018.
NASCENTE, A. S.; FILIPPI, M. C.; LANNA, A. C.; SOUZA, A. C. DE; SILVA LOBO, V. L. DE; SILVA,
G. B. Biomass, gas exchange, and nutrient contents in upland rice plants affected by application forms of microorganism growth promoters. Environmental Science and Pollution Research, v. 24, n. 3, p. 2956-2965, 2017. DOI: http://10.1007/s11356-016-8013-2
NAVEED, M.; QURESHI, M. A.; ZAHIR, Z. A.; HUSSAIN, M. B.; SESSITSCH, A.; MITTER, B. L-Tryptophan-dependent biosynthesis of indole-3-acetic acid (IAA) improves plant growth and colonization of corn by Burkholderia phytofirmans PsJN. Annals Of Microbiology, [S.L.], v. 65, n. 3, p. 1381-1389, 2014. DOI: http://dx.doi.org/10.1007/s13213-014-0976-y.
Oliveira, A. S. Desempenho agronômico do milho segunda safra em função da cultura antecessora e métodos de inoculação com Azospirillum brasilense. 2019. Dissertação (Mestrado) - Universidade Federal de Goiás, Unidade Acadêmica Especial de Ciências Agrárias, Programa de Pós Graduação em Agronomia, Jataí-GO. 2019.
RAZA, F. A.; FAISAL, M. Growth promotion of corn by desiccation tolerant Micrococcus luteus-chp37 isolated from Cholistan desert, Pakistan. Australian Journal of Crop Science, v. 7, n. 11, p. 1693-1698, 2013.
SHAO, J.; XU, Z.; ZHANG, N.; SHEN, Q.; ZHANG, R. Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens SQR9. Biology And Fertility Of Soils, [S.L.], v. 51, n. 3, p. 321-330, 2014. DOI: http://dx.doi.org/10.1007/s00374-014-0978-8.
SILVA, C. F. B.; BRITO, T. L.; TANIGUCHI, C. A. K.; LOPES, L. A.; PINTO, G. A. S.; CARVALHO, A. C. P. P. Growth-promoting potential of bacterial biomass in the banana micropropagated plants. Revista Brasileira de Engenharia Agrícola e Ambiental, [S.L.], v. 22, n. 11, p. 782-787, 2018. DOI: http://dx.doi.org/10.1590/1807-1929/agriambi.v22n11p782-787.
SILVA, M. A.; NASCENTE, A. S.; FILIPPI, M. C. C.; LANNA, A. C.; SILVA, G. B. DA; SILVA, J. F. A. Individual and combined growth-promoting microorganisms affect biomass production, gas exchange and nutrient content in soybean plants. Revista Caatinga, [S.L.], v. 33, n. 3, p. 619-632, set. 2020. DOI: http://dx.doi.org/10.1590/1983-21252020v33n305rc.
SOUSA, I. M.; NASCENTE, A. S.; FILIPPI, M. C. C. Bactérias promotoras do crescimento radicular em plântulas de dois cultivares de arroz irrigado por inundação. Colloquium Agrariae, [S. l.], v. 15, n. 2, p. 140–145, 2019. doi: http://10.5747/ca.2019.v15.n1.a293
SOUSA, S. M.; OLIVEIRA, C. A.; ANDRADE, D. L.; CARVALHO, C. G.; RIBEIRO, V. P.; PASTINA, M. M.; MARRIEL, I. E.; LANA, U. G. P. Cepas de Bacillus e Azospirillum aumentam o crescimento e a absorção de nutrientes em milho em condições hidropônicas. Sete Lagoas: Embrapa Milho e Sorgo. 2018.
SPAEPEN, S.; DOBBELAERE, S.; CROONENBORGHS, A.; VANDERLEYDEN, J. Effects of Azospirillum brasilense indole-3-acetic acid production on inoculated wheat plants. Plant And Soil, [S.L.], v. 312, n. 1-2, p. 15-23, 2008. DOI: http://dx.doi.org/10.1007/s11104-008-9560-1.
SPERANDIO, E. M.; VALE, H. M. M.; REIS, M. S.; CORTES, M. V. C. B.; LANNA, A. C.; FILIPPI, M. C. C. Evaluation of rhizobacteria in upland rice in Brazil: growth promotion and interaction of induced defense responses against leaf blast (Magnaporthe oryzae). Acta Physiologiae Plantarum, [S.L.], v. 39, n. 12, p. 258-270, 2017. DOI: http://dx.doi.org/10.1007/s11738-017-2547-x.
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